About The Xerra

The Xerra is a next generation cryo-fluorescence tomography tool that allows for comprehensive 3D fluorescence & anatomical images from 2D images. The Xerra does not require any radio-labeling and is located only inside the ARCF facility.

Features and Benefits

  • Superior resolution down to 20 um and up to 100 um in sections from 10 um to 50 um in thickness

  • High sensitivity to detect signals deep in tissue with an optical field of view of maximum 24 x 14 cm and minimum of 8 x 5 cm

  • High throughput, with the capacity to process several mice or multiple dissected organs on the same block

  • Reduces data variability by combining CFT with in vivo imaging and tissue microscopy from the same animal for reconstruction of whole body/tissue data in white and fluorescent light images

  • Streamlined user process, with no fixation, perfusion, tissue clearing, or radio-labeling required

  • Ability to select specific slices and transfer onto histology tape for further analysis

  • Maintains complete anatomical context with a single machine

  • Allows for versatile visualization from 470-780 nm

  • Automated workflows that obtain automated reconstructions of 2D slices into 3D as well as map exact anatomical location of molecular imaging data

  • Has integrated software and an onboard computer that allows for fast quantitative analysis, import/export function and provides access to open source data that can be integrated with iPACS

Applications

  • Imaging/characterization of tumor environment

  • Drug characterization

  • ADME information and visualization of whole animal

  • High resolution visualization of cell clusters, isolated organs and whole animals

  • Visualization of vector distribution & vector mediated gene expression

If you are interested in using the Xerra, please contact Darshini for more information about pricing.

Contact

Darshini Vijayakumar

Research Scientist I | University of Washington

Department of Radiology | Imaging Research Laboratory

Lab Manager | Radiology Optical Imaging Core

Portage Bay Building, Room 222

Seattle, WA 98101

dvijay26@uw.edu